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antibacterial activity against e coli atcc 25922  (ATCC)


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    ATCC antibacterial activity against e coli atcc 25922
    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
    Antibacterial Activity Against E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 49383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A next-generation probiotic strain for gut health: Bacteroides cellulosilyticus LYH2 variant with anti-inflammatory and metabolic advantages"

    Article Title: A next-generation probiotic strain for gut health: Bacteroides cellulosilyticus LYH2 variant with anti-inflammatory and metabolic advantages

    Journal: eBioMedicine

    doi: 10.1016/j.ebiom.2026.106232

    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of E. coli , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
    Figure Legend Snippet: Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of E. coli , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).

    Techniques Used: Bacteria, Cell Culture, Two Tailed Test, Control, Comparison, Co-Culture Assay, Evaporation



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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of <t>E.</t> <t>coli</t> , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).
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    Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of E. coli , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).

    Journal: eBioMedicine

    Article Title: A next-generation probiotic strain for gut health: Bacteroides cellulosilyticus LYH2 variant with anti-inflammatory and metabolic advantages

    doi: 10.1016/j.ebiom.2026.106232

    Figure Lengend Snippet: Metabolite profile of B. cellulosilyticus LYH2 and demonstration of the antimicrobial potential of its metabolites in co-cultivation with pathogenic bacteria. (A) Production of SCFAs by B. cellulosilyticus LYH2 cultured in BHI medium. Quantified SCFAs include AA, PA, BA, IBA, VA, and IVA. Data are presented as mean ± SEM. Asterisks denote statistical significance (∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001) as determined by unpaired two-tailed Student's t -test (∗ P < 0.05). Isovalerate was detected exclusively in the B. cellulosilyticus LYH2 group and was below the limit of detection in the BHI control. Therefore, no statistical comparison was applicable. (B–C) The volcano plots visually represent the magnitude fold change (|log2FC| > 1) and statistical significance (FDR < 0.05) of metabolites in positive and negative ion modes, contrasting their abundance in Ce and BHI media (n = 3). (D–E) The Principal Component Analysis (PCA) plots depict the clustering of samples based on positive and negative ion mode metabolite profiles in BHI and Ce media. Distinct colours represent separate groups. (F) A bar plot presents the enrichment of KEGG pathways, comparing the metabolic differences between Ce and BHI media (n = 3). (G) The preparation and subsequent co-culture process of B. cellulosilyticus LYH2 metabolites. (i) Metabolite solids are obtained via nitrogen evaporation under high-purity nitrogen conditions. (ii) Comparison of bacterial culture concentrations before and after the addition of B. cellulosilyticus LYH2 metabolites. The tubes on the left and right serve as positive controls containing pathogens, while the middle tubes represent the metabolite treatment group. (H–J) Growth curves of E. coli , S. aureus , and S. T yphimurium with and without the addition of metabolites (n = 3).

    Article Snippet: Antibacterial activity against E. coli ATCC 25922, S. aureus ATCC 25923, and S. T yphimurium ATCC 14028 was assessed by co-culturing, with detailed methods provided in the .

    Techniques: Bacteria, Cell Culture, Two Tailed Test, Control, Comparison, Co-Culture Assay, Evaporation